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Calculate the recovery by comparing the measured value with the expected amount of GSTM1 in the sample

Calculations from immunolocalization studies performed in parallel with whole tissue quantification yielded estimated glutathione concentrations of 1 to 6 mM in the plastids, cytosol, and mitochondria of Arabidopsis Col-0 leaf mesophyll cells, with concentrations in the vacuole below 50 M (Queval et al., 2011)

In studies involving delicate cellular systems or tissue samples, this solution is particularly valuable for ensuring the accuracy of dilution processes and maintaining consistency across experimental replicates

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Instead, let the vial rest for 10 to 20 minutes or use sonication if available