Exonuclease VII, 250 U @ 10 U/uL ISOBUC Avoid T1 and T5 phage

Avoid T1 and T5 phage contamination with tonA mutation

Addition of a poly(A) tail to an RNA molecule or a mixture of RNA molecules in order to provide a priming site for synthesis of first-strand cDNA using a primer with poly(dT) on its 3´ end

Improved success in demanding downstream applications

Unit Concentration 50

> 1 × 1010 cfu/µg pUC DNA)

Exonuclease VII, 250 U @ 10 U/uL ISOBUC Avoid T1 and T5 phageDigest duplex DNA in a 3 to 5 direction from a nick, blunt end, or 3 recessed end, producing stretches of ssDNA. Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' ends Protect protruding 3' ends becuase this exonuclease will not start digestion at protruding 3' overhangs Applications Production of intermediates for site directed mutagenesis. 1 3 Production of strand specific radiolabeled probes. 4 Exonuclease