Rec J Exonuclease, 250 U @ 10 U/uL GFRNASC prior to removing and sealing

prior to removing and sealing the tube

Cold Spring Harbor Laboratory Press

and Vogelstein

greatly simplifying reaction optimization and setup

Minimises the possibility of cloning or sequencing contaminating chromosomal DNA from plasmid

Rec J Exonuclease, 250 U @ 10 U/uL GFRNASC prior to removing and sealingMagnesium dependent digestion of single stranded DNA in a 5 to 3 direction Specific: Digests ssDNA in the 5' to 3 direction Heat Inactivated: Heat at 65C for 20 minutes to kill enzyme activity Flexible: Use to remove primers from completed PCR or to degrade single stranded linear DNA in dsDNA and plasmid preps Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5 to 3 direction. Its activity is dependent on Mg2+. Rec J