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Following treatment, cells are exposed to the Caspase-Glo3/7 (Promega) reagent for 30 minutes then fluorescence is read on a plate reader

A laboratory providing full nasal spray testing needs: laser diffraction instrumentation (Malvern, Sympatec, or equivalent) for DSD

PXR activation leads to repression of bile acid synthesis due to its physical association with hepatocyte nuclear factor 4 (HNF-4) causing this transcription factor to no longer be able to associate with the transcriptional co-activator PGC-1 (PPAR co-activator 1) which ultimately leads to loss of transcription factor activation of CYP7A1

OTC serums often label 1% peptide blend, but actual GHK-Cu is a fraction of that

It should not be attempted in non-medical settings