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Beating hearts were rapidly excised, cannulated, and perfused in isovolumic Langendorff mode with KrebsHenseleit (KH) buffer continuously gassed with 95% O 2 /5% CO 2 (pH 7.4, 37 C) containing (in mM): NaCl (116), KCl (4.7), MgSO 4 .7H 2 O (1.2), NaHCO 3 (25), CaCl 2 (1.4), and glucose (11) 60,61,62

& Meng, H

Briefly, a grown culture of Muller Hinton broth with pure bacterial colonies (1.0 10 6 CFU/mL) was inoculated with ascorbic acid at MIC and double MIC

We utilized molecular modeling approaches, activity assays using recombinant GLRX, coupled with site-directed mutagenesis of each cysteine both individually and in combination to address the oxidizibility of GLRX cysteines