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Recordings were obtained using a Multiclamp 700B amplifier (Molecular Devices, Sunnyvale, CA), Analog signals were low-pass Bessel filtered at 2 kHz, digitized at 10 kHz through a Digidata 144 OA interface (Molecular Devices), and stored in a computer using Clampex 10.2 software (Molecular Devices)

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For example, if you mixed 2 mL of BAC water into a 10 mg vial (creating 5 mg/mL), a 1 mg dose equals 20 units on an insulin syringe