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The double role of p53 in cancer and autoimmunity and its potential as therapeutic target

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Samples were then dehydrated in a gradient of ethanol concentrations and acetone, and then embedded in resin

Human tumor cell lines are obtained from ATCC (e.g., MCF7, U-87 MG, MDA-MB- 468, PC-3), grown to confluency in T75 flasks, trypsinized with 0.25% trypsin, washed one time with Tumor Cell Media (DMEM + 10%FBS), and plated in 90 at 5,000 cells/well in Tumor Cell Media

These systems can deliver far more of a drug to the brain than the aerosolized or atomized solutions currently available