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IF and IHC staining of tissue sections Tumour tissue sections were deparaffinized in Histo-Clear (Scientific Laboratory Supplies) for 5 min, rehydrated through a series of decreasing ethanol concentrations, followed by a final 5 min wash in distilled water (dH 2 O)

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Among its pivotal regulatory nodes, the Vitamin DVitamin D receptor (VDR) signaling axis acts as an integrative hub that senses and coordinates photic, redox, and metabolic cues to regulate immune homeostasis and structural integrity, thereby shaping the skins defensive and reparative capacity throughout aging

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