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Briefly, MDA can react with thiobarbituric acid (TBA) to generate the MDA-TBA adduct which can be measured spectrophotometrically at 532 nm

Schoenenberger (1986) Delta sleep-inducing peptide in spontaneously hypertensive rats Pharmacology Biochemistry and Behavior, Volume 24, Issue 6, June 1986, Pages 1797-1799

Eisenreich, W., Rudel, T., Heesemann, J

At a molecular level, smoking affects key signaling pathways such as nuclear factor-kappa B (NF-kB) and mitogen-activated protein kinases (MAPK) and induces epigenetic modifications like DNA methylation and histone modification [22]

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