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Shaking creates foam at the air-liquid interface, which denatures molecules at the boundary

The duration of immobility was measured by an observer blind to the treatment conditions (Porsolt et al., 1978) after treatment with vehicle, only cannabis (5, 10 or 15 mg/kg, s.c.), fluoxetine, sertraline, imipramine or after cannabis (5 mg/kg) co-administered with the antidepressant drugs

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In addition, excessive zinc can inhibit the absorption of selenium, affecting the activity of selenium-containing enzymes, e.g., glutathione peroxidase, which is important for antioxidant defense in the body

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