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Cells were washed thrice with phosphate-buffered saline and treated with dimethyl sulfoxide or compounds in Dulbecco's modified Eagle medium (high glucose) without fetal bovine serum in the presence of mitomycin C (1 M)

For lower glutathione concentrations, such as in blood samples, longer incubation times are required

The severity of lesions in neurons further progressed after a 3 hour period leading to additional worsening, noted at the final 24 hour post-paracetamol point ( Table 7, Table 8, Fig

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