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Reverse transcription of 1 g of RNA was performed with the HiScript III All-in-one RT SuperMix Perfect for qPCR (Vazyme, R333)

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Waist measurements typically decrease by 0.5-1.5 inches during this window, depending on the protocol and starting point

When injected subcutaneously, it binds to the GHRH receptor on the pituitary and triggers a pulse of growth hormone release