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Swelling: The swollen blood vessels may be felt as a lump or blockage in the anus

The quantification was set to Robust LC (high precision) mode with normalization disabled, MBR enabled, protein inferences set to Genes and Heuristic protein inference turned off

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Then, 1.5 mL of extraction buffer (80% methanol with 0.1% formic acid, v/v) was added, with vortexing for sample resuspension

In mammals, ferroptosis has been implicated in diverse pathologies, including neurodegenerative diseases, stroke, traumatic brain injury and kidney degeneration, as well as in carcinogenesis, where it may exert a tumor-suppressor function