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Shikonin activity can be inhibited by the inclusion of N-acetyl-L-cysteine, a known antioxidant and a precursor to GSH, which neutralizes ROS as part of the alternate glutathione synthase pathway, or through the over expression of TrxR itself (Duan et al., 2014)

Collectively, these preclinical findings demonstrate that melatonin bears multifaceted anticancer properties, ranging from direct tumor suppression and apoptosis induction to immune modulation and overcoming chemotherapy resistance

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