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An additional 50uL of NMR buffer was added to the filter, centrifuged again to wash, and combined for a total 250 L of sample that was transferred to a 3 mm NMR tube with a Teflon cap and sealed with parafilm

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Current research indicates that the cell cycle arrest depends on the activity of cyclin-dependent kinase inhibitors (CDK) p16 and p21, often regulated by the main tumor suppressor protein p53, whose elevated level is frequently used as a marker of senescence detection [21]