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(C) Safranin O/Fast green staining

The chemical equation for condensation reaction is as follows: C 6 H 5 N 3 O + C 11 H 16 F 6 N 5 OP + C 5 H 11 NO + C 7 H 7 NO 2 + C 10 H 18 O 5 -R1-NH 2 C6H5N3O + C 11 H 16 F 6 N 5 OP + C 5 H 11 NO + C 7 H 7 NO 2 + C 10 H 18 O 5 -R1-NH 2 After the condensation reaction is completed, the protective group of the amino acid needs to be removed to obtain the target peptide chain

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Acetochlor is known to cause lactate accumulation mediating necroptosis, yet interferes with Warburg-like effects to effectively attenuate necroptosis (183)