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The lipid residue in the LC-MS injection vial was redissolved with 300 L of isopropanol-methanol (V:V=1:1), vortexed for 30 s, and sonicated with ice water for 3 min, then the solution was transferred to a 1.5 mL centrifuge tube, and left to stand at -20C for 2 h

Increased immunity and antioxidant defense

Stevens et al., 1988

Food and Drug Administration (FDA) took this into account and still deemed hydroquinone safe for topical application because not enough clear evidence of the risk outweighing the benefits exists