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Its performance characteristics include: Glutathione coupled to Sepharose High Performance, excellent for high resolution purification of GST-tagged proteins Easy one-step purification of GST-tagged proteins and other glutathione S-transferase or glutathionedependent proteins Prepacked GSTrap HP columns for simple operation with a syringe, pump or chromatographic system, such as KTAdesign These features make Glutathione Sepharose High Performance the ideal medium for high resolution purification of GST-tagged proteins, glutathione S-transferases and glutathione binding proteins

90 L of the cell culture was added into each well containing 10 L of the azido-tagged dye at 500 M in 96-well plates, covered with plastic film, and incubated for 1 h at 37 C in a shaker incubator

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