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The study was conducted on 32 male mice divided into four groups: a control (C) group receiving a standard diet with biotin, a biotin-deficient (BD) group on a biotin-free diet, a ketogenic control (KC) group on a ketogenic diet (low-carbohydrate and high-fat) with biotin, and a ketogenic biotin-deficient (KBD) group on a ketogenic diet without biotin

Ethical statement All animal care and experimental protocols for in vivo studies were performed in accordance with the Guide for the Care and Use of Laboratory Animals, published by the National Institutes of Health (NIH

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doi: 10.1080/17425255.2017.1251580 57 IsholaIOBen-AzuBAdebayoOAAjayiAMOmorodionILEdjeKEet al