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In vitro studies confirm minimal cytotoxicity with no impact on cell viability up to 100 M concentrations across multiple cell types, though higher concentrations may produce non-specific effects that could confound experimental results

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L-mimosine (0253), aphidicolin (A0781), nocodazole (M1404), colchicine (C9754), H89 dihydrochloride hydrate (B1427), Forskolin (F6886), and Dimethyl sulfoxide (DMSO) were purchased from Sigma (Santa Clara, CA, USA)