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This finding supports the idea that the mutation in pad2-1 close to the glutamate conjugating residues of GSH1 might influence the affinity of the enzyme for BSO, as it was proposed for cad2-1 54,71
DSIP had no sleep-inducing effect within the first hour post-injection, but other effects on sleep, such as a significant decrease in the percentage of stage I sleep, reduced awakenings, and increased total sleep time, were significant (t-test), although not significantly from the second to the sixth hour